。html

Chromogenic Endotoxin Quantification Kit

Endotoxins, also known as lipopolysaccharides (LPS), are a major component of the outer membrane of Gram-negative bacteria. Their presence in pharmaceuticals, medical devices, and other biological products can lead to severe inflammatory responses in humans. Accurate detection and quantification of endotoxins are critical for ensuring product safety and compliance with regulatory standards.

What is the Chromogenic Endotoxin Quantification Kit?

The Chromogenic Endotoxin Quantification Kit is a highly sensitive and reliable tool designed for the quantitative measurement of endotoxin levels in various samples. This kit utilizes a chromogenic substrate that reacts with endotoxins in the presence of Limulus Amebocyte Lysate (LAL), producing a color change proportional to the endotoxin concentration.

Key Features of the Kit

• High Sensitivity: Capable of detecting endotoxin levels as low as 0.005 EU/mL.
• Wide Dynamic Range: Suitable for a broad range of endotoxin concentrations.
• Rapid Results: Provides accurate measurements within 30-60 minutes.
• Easy to Use: Simple protocol with minimal sample preparation.
• Regulatory Compliance: Meets USP, EP, and JP standards for endotoxin testing.

Applications of the Chromogenic Endotoxin Quantification Kit

This kit is widely used in various industries and research fields, including:

• Pharmaceuticals: Testing raw materials, intermediates, and final products.

Keyword: Chromogenic Endotoxin Quant Kit

• Medical Devices: Ensuring the safety of implants and other devices.
• Biotechnology: Monitoring endotoxin levels in cell cultures and recombinant proteins.
• Water Quality: Detecting endotoxins in drinking water and industrial water systems.

How the Kit Works

The chromogenic assay involves the following steps:

1. Sample Preparation: Dilute the sample if necessary to fall within the kit’s detection range.
2. Reaction Setup: Mix the sample with LAL reagent and chromogenic substrate.
3. Incubation: Allow the reaction to proceed at 37°C for a specified time.
4. Measurement: Measure the absorbance at 405 nm using a microplate reader.
5. Data Analysis: Compare the absorbance values to a standard curve to determine endotoxin concentration.

Advantages Over Other Methods

Compared to traditional methods like the gel-clot assay, the chromogenic endotoxin quantification kit offers several advantages:

• Quantitative Results: Provides precise numerical data rather than qualitative endpoints.
• Higher Throughput: Suitable for testing multiple samples simultaneously.
• Reduced Variability: Minimizes subjective interpretation of results.

Conclusion

The Chromogenic Endotoxin Quantification Kit is an essential tool for industries and researchers requiring accurate and reliable endotoxin detection. Its high sensitivity, ease of use, and compliance with regulatory standards make it a preferred choice for ensuring product safety and quality.</